How individuals are located in space can impact their interactions. We investigated spacing behavior of tadpoles of Gray Treefrogs (Hyla versicolor) and Green Frogs (Rana clamitans) at two densities in the laboratory. Tadpoles of H. versicolor were more dispersed than those of R. clamitans. Spacing (e.g., random, dispersed, or aggregated) of tadpoles was not affected by density; however, dispersion tended to increase during the experiment. Our results suggest that spacing behavior in tadpoles is dynamic over time and may provide some insight into the outcome of intra- and interspecific interactions.