A diminutive new species of Uperoleia (male snout–vent length, SVL, 17.3–21.3 mm female, SVL 21.8 mm) is described from 30 km south of Darwin, Northern Territory, Australia. The combination of small size, possession of maxillary and premaxillary teeth, and a completely exposed frontoparietal fontanelle distinguish the species from all other Uperoleia. The variation in number of pulses and duration of the advertisement call distinguish the species from the other sympatric Uperoleia species, Uperoleia inundata and Uperoleia lithomoda, as well as from the other Northern Territory species. The proximity of the type locality to Darwin highlights the current inadequate state of knowledge of the northern Australian frog fauna.