A new species of Stefania (Anura: Cryptobatrachidae) from northern Amazonas, Brazil, is described. Three specimens were collected in the Parque Nacional do Pico da Neblina, municipality of São Gabriel da Cachoeira, Amazonas State, northern Brazil, at 110–850 m elevation in dense closed canopy forest and in dense closed canopy montane forest. The new species is characterized by head length slightly greater than width, canthus rostralis concave, toes more than 50% webbed, dorsal skin smooth or shagreened. This is the first record of a species of Stefania endemic to Brazil.