Prior to both offensive and defensive striking, snakes can display notable differences in prestrike behaviors between offensive and defensive contexts. However, few studies have investigated strike movements during the different scenarios with which snakes are faced. To better understand how snakes strike, we measured the strikes of Western Ratsnakes (Pantherophis obsoletus; N = 11) presented with two different targets: one simulated predator (a gloved human hand) and one prey (pre-killed mice). For each strike, we recorded strike distance, duration, velocity (average and peak), acceleration (average and peak), and time to start mouth gape. In both encounters, ratsnakes displayed similar time to the initiation of a mouth gape while all peak performances were significantly different between strike types with performances being higher in defensive strikes. Defensive strikes took longer (mean = 122 ± 13 ms), reached greater distances (mean = 15.1 ± 1.7 cm), had higher maximum velocities (mean = 1.80 ± 0.11 ms^-1), and maximum accelerations (mean = 101.4 ± 15.2 ms^-2). Offensive strikes had much shorter durations (mean = 49 ± 5 ms), distances (mean = 4.3 ± 0.6 cm), maximum velocities (mean = 1.06 ± 0.10 ms^-1), and maximum accelerations (mean = 81.4 ± 18.9 ms^-2). The results for average performance measurements are similar to those for the maximum performance comparisons. Our results show that snakes can recognize and differentiate prey from threats and respond differently in each situation. Our results also show that predatory and defensive strikes are quantitatively and situationally distinct, should be treated as separate behaviors, and therefore should be evaluated and analyzed separately from one another.