Min Li, Qiang Liu, Li Xi, Yang Liu, Gengping Zhu, Yanni Zhao, Wenjun Bu
Journal of Insect Science 14 (79), 1-11, (1 May 2014) https://doi.org/10.1673/031.014.79
KEYWORDS: 16s rDNA, COI, Cyt b, molecular markers
The COI gene as the core of the DNA barcoding system for animals has received significant attention. The observed wide overlap between intra- and interspecific sequence variability has led researchers to envisage the primary COI-based method. The sequences of 16S rDNA, COI, and Cyt b genes of Nezara virudula (L.) (Hemiptera: Pentatomidae) from 13 countries and the same sequences of N. antennata Scott were chosen as molecular markers to analyze the intra- and interspecific relationships between the closely related species in this study. The results support that Cyt b gene may be a good candidate alongside COI, when the combined factors of geographic variation and closely related species are taken into account.