Deryck R. Persaud, Norbert H. Haunerland
Journal of Insect Science 4 (6), 1-10, (1 February 2004) https://doi.org/10.1673/031.004.0601
KEYWORDS: Storage protein receptor, receptor mediated endocytosis, arylphorin, very high density lipoprotein, basic juvenile hormone suppressible protein, hexamerin, insect metamorphosis
In Noctuids, storage proteins are taken up into fat body by receptor-mediated endocytosis. These include arylphorin and a second, structurally unrelated very high-density lipoprotein (VHDL). Previously, we have isolated a single storage protein receptor from the corn earworm, Helicoverpa zea, which binds both VHDL and arylphorin. The receptor protein is a basic, N-terminally blocked, ∼80 kDa protein that is associated with fat body membranes. Microsequencing of proteolytic fragments of the isolated receptor protein revealed internal sequences that were used to clone the complete cDNA of the VHDL receptor by 3′ and 5′ RACE techniques. The receptor protein, when expressed in vitro via a suitable insect expression vector, reacted with antibodies against the native VHDL receptor and bound strongly to its ligand VHDL, thus confirming that the cloned cDNA represents indeed the previously purified VHDL receptor. The receptor protein and a second, similar protein also found associated with the fat body membrane show considerable homology to putative basic juvenile hormone suppressible proteins cloned previously from other Noctuid species. Sequence analysis revealed that the receptor is likely a peripheral membrane protein that may mediate the selective uptake of VHDL.
basic juvenile hormone suppressible protein
rapid amplification of cDNA ends
very high density lipoprotein