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1 April 2005 Evidence-based Embryo Cryopreservation
Masashige Kuwayama
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Abstract

In 1972, cryopreservation of mammalian animal embryos was developed using mouse embryos. To utilize surplus embryos for in vitro fertilization programs, this technique was applied to human embryos by the slow freezing method in the first half of the 1980's, and by vitrification in the 1990's. Recently, the protocol of vitrification of human embryos has been improved by the ultra-rapid vitrification method in which the rate of cooling is highly improved. It is mainly used as an effective basic technique for cryopreservation of human embryos in current clinical IVF programs due to the extremely high embryonic survival rate after storage.

Masashige Kuwayama "Evidence-based Embryo Cryopreservation," Journal of Mammalian Ova Research 22(1), 28-32, (1 April 2005). https://doi.org/10.1274/jmor.22.28
Received: 18 January 2005; Accepted: 1 February 2005; Published: 1 April 2005
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KEYWORDS
cryopreservation
Human embryo
IVF
Slow freezing
vitrification
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