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1 October 2006 Freeze-dried Spermatozoa and Freeze-dried Sperm Injection into Oocytes
Hiroshi Suzuki
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Abstract

Attempts to freeze-dry spermatozoa are not new. Successful conception and full-term development using freeze-dried spermatozoa were reported in the cow and rabbit prior to 1960. However, these preliminary results have not been confirmed. Spermatozoa become defective in motility after freeze-drying and are unable to fertilize eggs both in vivo and in vitro. However, in 1998, freeze-drying of mammalian spermatozoa was demonstrated without loss of genetic or reproductive potential. Freeze-dried spermatozoa support normal development when injected directly into oocytes by means of an intracytoplasmic sperm injection (ICSI) technique. Recent investigations of freeze-dried sperm have focused on the factors affecting freeze-drying in spermatozoa in an effort to make it possible to store the male gamete at ambient temperature. The pressure level at primary dryied of freeze-drying spermatozoa, in addition to the components of the suspending solution, appears to be an important factor for long-term preservation of freeze-dried spermatozoa.

Hiroshi Suzuki "Freeze-dried Spermatozoa and Freeze-dried Sperm Injection into Oocytes," Journal of Mammalian Ova Research 23(3), 91-95, (1 October 2006). https://doi.org/10.1274/jmor.23.91
Received: 1 May 2006; Accepted: 1 May 2006; Published: 1 October 2006
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