To investigate the effect of media on mouse follicular development and oocyte maturation in vitro, we compared eight culture media. Early preantral follicles were mechanically dissected from the ovaries of 14-day-old mice and cultured for 10 days. The tested media were: α-MEM, D-MEM/F-12, D-MEM with high glucose (4.5 g/L) (D-MEM[H]) or low glucose (1 g/L) (D-MEM[L]), Weymouth, M199, IMDM, and RPMI1640. All of the media were supplemented with 5% FBS, ITS, 100 mlU/ ml rhFSH, 1 mlU/ml rhLH and, 0.5% gentamicin. Compared to the other media (P < 0.05), a higher percentage of follicles survived and antral-like cavity formation was seen after 10 days of culture when the follicles were cultured in α-MEM, Waymouth, D-MEM/F-12, or D-MEM(L). Among the four media with higher follicle survival, the oocyte diameter on day 10 of culture was largest in α-MEM and smallest in Waymouth. The highest percentages of MII oocytes were obtained when follicles were cultured in α-MEM, D-MEM(L), and D-MEM/F-12. In contrast, in Waymouth, no MII oocytes were obtained. The media used for mouse preantral follicle growth in vitro affected follicle survival and oocyte maturation. Of the media that we tested, α-MEM, D-MEM(L), and D-MEM/F-12 were superior for mouse preantral follicle cultures.
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Vol. 27 • No. 1