Cryopreservation of embryos is an important strategy for the conservation of species and valuable strains of laboratory species. Historically, in the mouse, the technical development of cryopreservation started with slow freezing methods in the 1970's, which was then followed by vitrification method developed in the 1980's. Vitrification is advantageous in its quickness and simplicity, because it does not need programmable controlled-rate freezing machines. Furthermore, the survivability of embryos recovered after vitrification is significantly improved by avoiding chilling injury and intra- and extra-cellular ice formation. Recently, a series of new vitrification methods using a minimal volume of cryopreservation agent (CPA) and extremely high osmolality CPAs have been developed. For laboratory rats and mice, there is a long history of embryo cryopreservation and huge numbers of embryos have been kept frozen in cryobanks. In contrast, the techniques have only recently been optimized for other laboratory animals, e.g., rabbits, Syrian hamsters, Mongolian gerbils and mastomys (African rodent). Besides safety in cryopreservation, simple transportation of vitrified embryos on dry ice has been a challenging issue. By using the extremely high osmolality CPAs, we are now examining the feasibility and effectiveness of a simple method for the transportation of vitrified embryos. Development of new cryopreservation methods for embryos of laboratory species should be an integral part of the technological logistics supporting the development of biomedical sciences.
You have requested a machine translation of selected content from our databases. This functionality is provided solely for your convenience and is in no way intended to replace human translation. Neither BioOne nor the owners and publishers of the content make, and they explicitly disclaim, any express or implied representations or warranties of any kind, including, without limitation, representations and warranties as to the functionality of the translation feature or the accuracy or completeness of the translations.
Translations are not retained in our system. Your use of this feature and the translations is subject to all use restrictions contained in the Terms and Conditions of Use of the BioOne website.
Vol. 27 • No. 3