The objective of the present study was to investigate the in vitro developmental competence of porcine in vitro matured (IVM) oocytes vitrified after removal of cytoplasmic lipid droplets (delipation). After vitrification and warming, the delipated porcine IVM oocytes were inseminated and subsequently cultured in vitro. The rate of development to the blastocyst stage of delipated, vitrified oocytes (5.9%) was significantly lower than that of control oocytes (untreated oocytes) (26.2%). We also examined the influence of delipation of porcine IVM oocytes on development to the blastocyst stage following in vitro fertilization (IVF). Delipated porcine IVM oocytes (not vitrified) were inseminated and subsequently cultured in vitro. The rates of development to the blastocyst stage were similar for delipated and undelipated oocytes (21.1% and 26.2%, respectively). The results of the present study showed that delipated, vitrified porcine IVM oocytes can develop to the blastocyst stage following IVF, though blastocyst formation rate was low, and that delipation of porcine IVM oocytes did not negatively affect their development to blastocyst stage.
You have requested a machine translation of selected content from our databases. This functionality is provided solely for your convenience and is in no way intended to replace human translation. Neither BioOne nor the owners and publishers of the content make, and they explicitly disclaim, any express or implied representations or warranties of any kind, including, without limitation, representations and warranties as to the functionality of the translation feature or the accuracy or completeness of the translations.
Translations are not retained in our system. Your use of this feature and the translations is subject to all use restrictions contained in the Terms and Conditions of Use of the BioOne website.
Vol. 30 • No. 1