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1 April 2014 Successful Pregnancy Following Transfer of Frozen—Thawed Day 7 Blastocysts Derived from Transported Oocytes
Taketo Inoue, Yoshiyuki Ono, Yukiko Yonezawa, Takuo Fujiwara, Ataru Nohara, Osamu Mimura, Junji Kishi, Nobuyuki Emi
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Abstract

Purpose: Pelvic adhesions inhibit follicular growth and ovum transport, causing female infertility. In this report, we describe successful ovum pick-up (OPU) during adhesiotomy in a woman with severe adhesions that prevented transvaginal OPU. Subsequently, in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) were attempted after transport of cumulus-oocyte complexes (COCs). Materials and methods: Clinical examination revealed a chocolate cyst on the patient's left ovary. Her right ovary was adherent to the posterior part of the uterus. COCs were harvested during adhesiotomy and stored at 37°C in a 1.5-ml tube containing Sperm Washing Medium. The tube was transported to our clinic using hot gel bags and a portable infant incubator. Immediately upon arrival, IVF and ICSI were performed. On day 7, two ICSI-derived blastocysts were cryopreserved. Two months later, these blastocysts were transferred to the patient's uterus. Results: The patient conceived, and a normal, healthy boy was born. Conclusions: In summary, we performed OPU during adhesiotomy, and COCs were transported to the infertility clinic on the same day; subsequently, the patient was successfully impregnated using the transported oocytes.

©2014 Japan Society for Ova Research
Taketo Inoue, Yoshiyuki Ono, Yukiko Yonezawa, Takuo Fujiwara, Ataru Nohara, Osamu Mimura, Junji Kishi, and Nobuyuki Emi "Successful Pregnancy Following Transfer of Frozen—Thawed Day 7 Blastocysts Derived from Transported Oocytes," Journal of Mammalian Ova Research 31(1), 52-56, (1 April 2014). https://doi.org/10.1274/jmor.31.52
Received: 25 June 2013; Accepted: 1 August 2013; Published: 1 April 2014
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KEYWORDS
blastocyst
cumulus-oocyte complex
Intracytoplasmic sperm injection
Pelvic adhesion
transportation
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