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1 October 2015 Effect of Artificial Oocyte Activation by Calcium Ionophore on One-Day-Old Unfertilized Oocytes after ICSI
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Abstract

At our clinic, a successfully fertilized human oocyte is defined as one with two polar bodies and two pronuclei (PN) 18 h after ICSI, but when some oocytes do not form PN. Recently, a successful pregnancy and live birth following artificial oocyte activation (AOA; using Ca2 ionophore) of ICSI-treated one-day-old unfertilized oocytes was reported. In this study, we performed AOA on PN-lacking unfertilized oocytes, 18 h after ICSI (defined as Day 1-AOA, since ICSI=Day 0). The abilities of these oocytes to form PN and develop were compared with those of oocytes fertilized by ICSI (defined as No-AOA). Piezo-ICSI was performed on 168 oocytes, 50 of which failed to fertilize (0PN) but were subsequently artificially activated. 2PN formation rate was significantly lower in Day 1-AOA, and the rates of 1PN and ≥4PN were significantly higher than in No-AOA. In Day 1-AOA, 8 oocytes formed 2PN, 6 zygotes underwent cleavage and 2 of them developed to the morula stage, but none formed blastocysts. The rates of cleavage, morula, and blastocyst formation were significantly lower in Day 1-AOA than in No-AOA. These results suggest that the AOA protocol has room for improvement in the activation of unfertilized oocytes 18 h after ICSI.

©2015 Japan Society for Ova Research
Masahiro Sakurai, Shinichi Watanabe, Toyomi Tanaka, Rie Matsunaga, Naoko Yamanaka, Minako Kamihata, Chikako Kani, Akiko Kuwahata, Masanori Ochi, and Toshitaka Horiuchi "Effect of Artificial Oocyte Activation by Calcium Ionophore on One-Day-Old Unfertilized Oocytes after ICSI," Journal of Mammalian Ova Research 32(3), 115-120, (1 October 2015). https://doi.org/10.1274/jmor.32.115
Received: 30 March 2015; Accepted: 1 July 2015; Published: 1 October 2015
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