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1 October 2016 Human Germline Development from Pluripotent Stem Cells in vitro
Naoko Irie, Shinseog Kim, M. Azim Surani
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Mammalian primordial germ cells (PGCs) are specified in the early post-implantation embryo. Attempts have been made to establish in vitro PGC development since the derivation of embryonic stem cells (ESCs) from blastocysts. Despite the advances made with mouse models, similar studies in human germ cell development have not progressed because practical and ethical reasons prevent the use of early human embryos. Recently, we and others developed a robust in vitro system for producing human primordial germ cell-like cells (hPGCLCs) from ESCs and induced pluripotent stem cells (iPSCs) by inducing competency for germ cells. Strikingly, the molecular mechanism for germline differentiation is not fully conserved between mouse and human, probably because of the differences in their early embryogenesis and regulation of the pluripotent state. Here, we present a review of the current status in the field of in vitro germ cell production from pluripotent stem cells, and discuss how its usefulness could be extended to clinical applications.

©2016 Japan Society for Ova Research
Naoko Irie, Shinseog Kim, and M. Azim Surani "Human Germline Development from Pluripotent Stem Cells in vitro," Journal of Mammalian Ova Research 33(2), 79-87, (1 October 2016).
Received: 5 April 2016; Accepted: 1 May 2016; Published: 1 October 2016

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human development
Human primordial germ cells
pluripotent stem cells
Primordial germ cell-like cells
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