The Kruger National Park (KNP) is home to the last genetically viable, minimally managed population of African wild dogs (Lycaon pictus, wild dogs) in South Africa. Until 2004, this population remained stable, but since has been declining. In this study, we aimed to improve our understanding of the ecology of KNP wild dogs by estimating the relative contribution of different prey types to their diet across landscape types. Based on a Bayesian mixing model, we assessed wild dog diet and foraging preferences using stable isotope analysis. We sampled 73 individuals from 40 packs found in six different landscape types. In thickets, packs predominantly prey on small browsing and mixed-feeding species (accounting for ∼73% of their diet), but occasionally hunt large grazers (∼24%) and large browsers (∼3%). In open landscape types where lions (Panthera leo) are more or less absent, such as in the Lowveld sour bushveld, wild dogs prey on large browsers and large grazers (∼67%). Our results demonstrate that KNP wild dogs occupy a broader ecological niche than previously thought, with small browsers forming an integral part of their diet. We also present the first data describing differences in wild dog diet–tissue discrimination factors for tail hair and whiskers compared to respective stable nitrogen (δ¹⁵N) and carbon (δ¹³C) values obtained from feces of captive wild dogs, as well as from those of South Africa's broader managed metapopulation. While these data should be considered preliminary, we suggest that until wild dog diet–tissue discrimination factors are calculated through a controlled feeding study, the discrimination factors calculated for the gray wolf (Canis lupus) should be used for wild dog-related isotope studies, rather than the often cited values for red foxes (Vulpes vulpes).

When colonizing new regions, invading species might compete strongly with phylogenetically related species native to the regions they are colonizing, eventually leading to coexistence or displacement. In the southeast of the United States, recently established coyotes (Canis latrans) compete with red fox (Vulpes vulpes) and gray fox (Urocyon cinereoargenteus), although it remains unclear if competition is leading to resource partitioning or displacement by species. Using nitrogen and carbon stable isotopes, we tested the hypothesis that coyotes compete with foxes for food resources, with canids partitioning those resources to mitigate competition. We compared diets of canids in the southeast to those in the Plains region of the United States, a region where all three species historically have coexisted. We analyzed 217 hair samples from both regions pre-1960, prior to coyote colonization of the southeast, and post-2000, after coyotes were ubiquitous there, to assess differences in diet among species for both regions (southeast versus Plains and time periods, pre- versus postcolonization by coyotes). Modeling revealed significant dietary overlap among historical and contemporary populations in the southeast. Historically, all species partitioned resources in the Plains. Contemporarily, red fox and coyotes co-occurring in the Plains overlapped in diet; however, gray fox diet did not overlap with those of red fox and coyotes. Absence of partitioning in diet among co-occurring canids in the southeast indicates that interspecific competition could be strong in the region. Competition among canid populations in the southeast could lead to further resource partitioning among species that promotes coexistence or competitive exclusion of smaller fox species where coyote populations are abundant.

Small mammal assemblages from South America provide a unique opportunity to measure coexistence and niche partitioning between marsupials and placentals. We tested how these two major clades partition environmental resources by comparing stable isotopic ratios of similar sized Didelphidae and Sigmodontinae in four Brazilian biomes: Pampas grassland, Pantanal wetland, Cerrado woodland savanna, and Atlantic Forest. Generally, didelphid isotopic niche follows a scaling law, because we found an association between δ¹⁵N enrichment and body mass. Sigmodontines that primarily partition the environment via forest strata showed a greater intake of C₄ or/and crassulacean acid metabolism (CAM) plants than didelphids, as reflected by their wider trophic niche. Values of δ¹³C were highest in savannas and grasslands (Cerrado and Pampas biomes), and values of δ¹⁵N were highest in the Atlantic Forest (in sigmodontines) and Pampas (in didelphids). While assessing patterns between the two major Brazilian biomes (Atlantic Forest and Cerrado), we found evidence of a broader trophic niche for both clades in the Cerrado. In the Atlantic Forest, niche occupation by Didelphidae was completely enclosed within the Sigmodontinae trophic niche. Both clades showed less overlap in the Cerrado, a less productive environment. Our results highlight the importance of a comparative framework and the use of stable isotopes for testing ecological questions related to how small mammalian communities partition their niche.

Habitat information for small mammals typically consists of anecdotal descriptions or infrequent analyses of habitat use, which often are reported erroneously as signifying habitat preference, requirements, or quality. Habitat preferences can be determined only by analysis of habitat selection, a behavioral process that results in the disproportionate use of one resource over other available resources and occurs in a hierarchical manner across different environmental scales. North American chipmunks (Neotamias and Tamias) are a prime example of the lack of studies on habitat selection for small mammal species. We used the Organ Mountains Colorado chipmunk (N. quadrivittatus australis) as a case study to determine whether previous descriptions of habitat in the literature were upheld in a multiscale habitat selection context. We tracked VHF radiocollared chipmunks and collected habitat information at used and available locations to analyze habitat selection at three scales: second order (i.e., home range), third order (i.e., within home range), and microhabitat scales. Mean home range was 2.55 ha ± 1.55 SD and did not differ between sexes. At the second and third order, N. q. australis avoided a coniferous forest land cover type and favored particular areas of arroyos (gullies) that were relatively steep-sided and greener and contained montane scrub land cover type. At the microhabitat scale, chipmunks selected areas that had greater woody plant diversity, rock ground cover, and ground cover of coarse woody debris. We concluded that habitat selection by N. q. australis fundamentally was different from descriptions of habitat in the literature that described N. quadrivittatus as primarily associated with coniferous forests. We suggest that arroyos, which are unique and rare on the landscape, function as climate refugia for these chipmunks because they create a cool, wet microclimate. Our findings demonstrate the importance of conducting multiscale habitat selection studies for small mammals to ensure that defensible and enduring habitat information is available to support appropriate conservation and management actions.

Understanding the mechanisms by which similar species coexist in sympatry is a major driver of ecological research. Niche partitioning and ecological plasticity can facilitate spatial and habitat use overlap between generalist and specialist species. Mouse lemurs (Microcebus spp.) are a highly speciose group of small primates that are endemic to the forests of Madagascar. In northwestern Madagascar, the relatively widespread M. murinus occurs sympatrically with the microendemic M. ravelobensis. We investigated spatial distributions and densities of these two species across a mangrove–dry forest habitat gradient in Mariarano commune. We used capture-mark-recapture techniques and nocturnal line transect surveys along six transects during June and July 2017. Spatial capture-recapture and distance sampling models were used to estimate lemur densities across habitat types. The congeners displayed differential patterns of spatial distribution and densities. Microcebus murinus was found in similar densities across all habitat types, while M. ravelobensis was found at much higher densities in dry forests compared with mangroves. This suggests that the generalist M. murinus uses a wider array of habitats more evenly than the specialist M. ravelobensis. Our study provides empirical evidence of how cryptic lemur species differ in their habitat use and distribution across an environmental gradient and provides new insights into their ecology in an understudied habitat. Lemurs are one of the most threatened groups of mammals in the world, and understanding how these species are distributed across different forest types is crucial for planning and implementing conservation measures to protect lemur habitat.

Two short-tailed opossum species, Monodelphis glirina and M. touan, occur in sympatry in an area of eastern Amazonia. Habitat structure and resource availability may influence habitat use and, consequently, species distribution, detectability, occupancy, and abundance. We evaluated occupancy and detectability of the species M. glirina and M. touan in the Carajás National Forest to answer the following questions: (1) Do both species occur in canga and forest habitats? (2) Do detectability and occupancy of M. touan and M. glirina differ in areas of canga and forest? (3) Does the presence of one species affect the detection of the other? We undertook surveys at 50 sampling sites (26 in canga and 24 in forest habitat). In addition, we developed co-occurrence models to test the relationships between occupancy and detection of M. touan in the presence or absence of M. glirina. We captured 693 individuals of M. glirina (587 in canga and 106 in forest) and 112 of M. touan (only one individual captured in canga). Occupancy by M. glirina was positively influenced by superficial rock cover and litter depth, while detectability was negatively influenced by canopy cover. Occupancy by M. touan was influenced positively by canopy cover, number of fallen trunks on the ground, and litter depth (Ψ = 0.315). Data from forest sites where M. touan and M. glirina occurred more often corroborated our third hypothesis, that detectability of M. touan is low when M. glirina is present. Our results highlight the existence of habitat preference by the two species of Monodelphis.

Utah prairie dogs (UPDs, Cynomys parvidens) are colonial, herbivorous rodents listed under the Endangered Species Act as threatened. Little is known about UPD population dynamics at higher elevations in the species' range. From 2013 through 2016, we studied UPDs on five colonies at 2,645 to 2,873 m elevation on the Awapa Plateau, Utah, USA. Primary production increases with precipitation and precipitation increases with elevation on the plateau. We hypothesized that UPD body condition, reproduction, survival, and population growth all would vary directly with precipitation and elevation. Each year, we live-trapped UPDs from late-Jun through Aug, weighing each UPD, aging it as adult or pup, measuring its right hind foot, marking it for unique identification, and releasing it at point of capture. Fleas from live-trapped UPDs and opportunistically collected rodent carcasses, and rodent carcasses themselves, were tested for the agent of sylvatic plague (Yersinia pestis), a lethal invasive pathogen. Adult UPD body condition (mass:foot) increased with elevation. In addition, UPD reproduction (pups:adults) and population growth (λ) increased with precipitation. Annual survival declined from 0.49 in 2013–2014 to 0.24 in 2015–2016. We captured 421 UPDs in 2013 but only 149 in 2016. Sylvatic plague may have contributed to population declines. Notwithstanding, plague detection (yes/no by colony and year) had no statistical effect on population growth or annual survival, raising suspicion about the predictive value of binary plague detection variables. Generally speaking, efforts to conserve UPDs may benefit from the restoration and preservation of large colonies at mesic sites.

Wild boar (Sus scrofa) is one of the most damaging invasive species in the world and can have a profound impact on the distribution of native species. Nevertheless, there still are limitations on the species' current fine-scale spatial information, which is needed to develop effective management measures. Here, we used Species Distribution Models (SDMs) and niche overlap analysis to estimate potential conflict areas between the wild boar and the native southern pudu (Pudu puda), which is a bioindicator of the forest conservation status within the Nahuel Huapi National Park (NHNP), Argentina. The two species' environmental niche overlaps by 40%, which results in a wide geographical overlap between wild boar and pudu distributions. The distribution model predicted that the wild boar potential distribution occupies 22% of the national park and overlaps up to 67% with the pudu distribution, which in turn occupies 20% of the park. Based on our models, we classified 12% of the park as extreme management priority areas, because both species are present. High priority areas, where wild boars have the potential to invade but will not overlap with pudu populations, represent 10% of the park. Medium priority areas, where wild boars do not threaten pudu populations, and low priority areas, with no potential presence of either species, are 8% and 68%, respectively. The results of this study show how SDMs developed at local scales can support the management and monitoring of native and invasive species and help guide the allocation of efforts and resources for management actions focused on protected areas.

Understanding the mechanisms by which alien species become invasive can assure successful control programs and mitigate alien species' impacts. The distribution of invasive wild pigs (Sus scrofa) has been sharply expanding throughout all regions of Brazil in the last few years. Here we demonstrate that large monocultural plantations provide the primary resource subsidies to invasive wild pigs in Brazil. We analyzed 106 stomach contents and carbon stable isotopes (δ¹³C) of 50 hair samples of wild pigs from a population immersed in a landscape dominated by sugar cane agriculture. Stomach contents were dominated by corn (41%), sugarcane (28.5%), vegetal matter (all other vegetation besides crops, 27%), and animal matter (vertebrates and invertebrates, 4%). Bayesian mixing model analysis of δ¹³C showed that food sources from C₄ photosynthetic pathway (represented by corn and sugarcane) accounted for 94% of the long-term diet, while C₃ food sources for only 6.2%. Our results indicate that corn and sugar cane are subsidizing the diet of wild pigs and can facilitate the population growth of this invasive species. Given that Brazil is a major agricultural producer and a hotspot of biodiversity, it is extremely concerning that extensive agriculture may accelerate the expansion of this invasive species, resulting in economic losses and cascading effects on natural habitats.

Efforts to remove invasive species may benefit native species, but the effects can be complex and unpredictable. Thus, studies of invasive-species removal provide important information for guiding management and providing insight about variation in post-removal impacts within the community. Using southern pine-grassland ecosystems as a model system, we hypothesized that removal of the long-established red-imported fire ant (Solenopsis invicta, hereafter RIFA) would positively influence altricial Peromyscus species, due to increased survival of young in the nest and thus increased recruitment to the population, but would not impact semi-precocial hispid cotton rats (Sigmodon hispidus), which are mobile more quickly after birth and thus at less risk of depredation by RIFA. We compared small mammal populations on sites treated with a granular insecticide (Extinguish Plus) to remove RIFA in southwestern Georgia, United States, from April 2018 to December 2019. As expected, we detected no difference in cotton rat recruitment. However, contrary to our prediction, the same was true for cotton mice (Peromyscus gossypinus) and oldfield mice (Peromyscus polionotus). We found RIFA removal increased survival both of cotton rats and cotton mice, increasing average population rate of change (λ) on treated sites during the study period. In contrast, we observed lower survival of oldfield mice, with similar λ estimates on treated and untreated sites, but low sample sizes were problematic for this species. Our results show that removal of invasive species can have positive impacts for native species, but both the magnitude of RIFA effects on small mammals and mechanisms by which impacts occur are complex.

Tricolored bats (Perimyotis subflavus) that roost in subterranean hibernacula have experienced precipitous declines from white-nose syndrome (WNS); however, understudied populations also use during winter non-subterranean roosts such as tree cavities, bridges, and foliage. Our objectives were to determine winter roost use by tricolored bats in an area devoid of subterranean roosts, determine roost microclimates to relate them to growth requirements of the fungal causal agent of WNS, and determine habitat factors influencing winter tree selection. From November to March 2017–2019, we used radiotelemetry to track 15 bats to their day roosts in the upper Coastal Plain of South Carolina and recorded microclimates in accessible tree cavities and bridges. We also characterized habitat and tree characteristics of 24 used trees and 153 random, available trees and used discrete choice models to determine selection. Roost structures included I-beam bridges, cavities in live trees, and foliage. Bridges were warmer and less humid than cavities. Roost temperatures often were amenable to fungal growth (< 19.5°C) but fluctuated widely depending on ambient temperatures. Bats used bridges on colder days (8.7°C ± 5.0 SD) and trees on warmer days (11.3°C ± 5.4). Bats selected low-decay trees closer to streams in areas with high canopy closure and cavity abundance. Bats also appeared to favor hardwood forests and avoid pine forests. Our results suggest that access to multiple roost microclimates might be important for tricolored bats during winter, and forest management practices that retain live trees near streams and foster cavity formation in hardwood forests likely will benefit this species. Our results also suggest tricolored bats using bridge and tree roosts might be less susceptible to WNS than bats using subterranean hibernaculum roosts. Thus, forests in areas without subterranean hibernacula in the southeastern United States that support bats during winter might represent important refugia from WNS for multiple species.

Animal migrations occur in many taxa and are considered an adaptive response to spatial or temporal variations in resources. Human activities can influence the cost-benefit trade-offs of animal migrations, but evaluating the determinants of migration trajectory and movement rate in declining populations facing relatively low levels of human disturbance can provide new and valuable insights on the behavior of wildlife in natural environments. Here, we used an adapted version of path selection functions and quantified the effects of habitat type, topography, and weather, on 313 spring migrations by migratory caribou (Rangifer tarandus) in northern Québec, Canada, from 2011 to 2018. Our results showed that during spring migration, caribou selected tundra and avoided water bodies, forest, and higher elevation. Higher precipitation and deeper snow were linked to lower movement rates. Weather variables had a stronger effect on the migration trajectories and movement rates of females than males. Duration of caribou spring migration (mean of 48 days) and length (mean of 587 km) were similar in males and females, but females started (22 April) and ended (10 June) spring migrations ca. 6 days earlier than males. Caribou spring migration was influenced by habitat type, topography, and weather, but we also observed that caribou migrations were not spatially constrained. Better knowledge on where and when animals move between their winter and summer ranges can help inform management and land planning decisions. Our results could be used to model future migration trajectories and speed of caribou under different climate change scenarios.

Understanding how demographic parameters respond to climatic variables is essential for predicting species' response to changing environmental conditions. The California pocket mouse (Chaetodipus californicus) is an inhabitant of coastal-central California oak (Quercus spp.) woodland that is undergoing a rapid anthropogenic transformation while also facing effects of global climate change. We analyzed the population dynamics of the California pocket mouse by applying Pradel's temporal symmetry model to a 10-year (2004 – 2013) capture–mark–recapture data set to estimate survival and recruitment rates and realized population growth rate. The overall monthly apparent survival probability (ϕ) was 0.76 ± 0.01 SE and was slightly higher in the dry season (0.79 ± 0.02 SE) than the wet season (0.74 ± 0.01 SE). Coefficients of variation (CV) of temperature and rainfall (with and without a one-season lag), average seasonal temperature, and regional climatic variation (El Niño index) positively influenced ϕ. Overall monthly recruitment rate (f) was 0.17 ± 0.01 SE but varied seasonally; f was substantially higher during the dry season (0.39 ± 0.04 SE) than the wet season (0.09 ± 0.02 SE). Average seasonal temperature, CV of temperature and rainfall (without a one-season lag), and total seasonal rainfall (with a one-season lag) positively influenced recruitment, whereas regional climatic variation (El Niño index), total seasonal rainfall (without a one-season lag), and CV of rainfall (with a one-season lag) had a negative effect on f. Monthly realized population growth rate (λ) was 1.00 ± 0.02 SE for the entire study period, but it varied temporally. Our study provides the first estimates of demographic parameters for the California pocket mouse and tests for the influence of climatic variables on these parameters. Although the California pocket mouse population remained relatively stable during our study (as indicated by λ = 1.00), changing climate and anthropogenic influences on California oak woodland could adversely influence demographic parameters and population dynamics and might also indicate effects of climate change on its ecologically sensitive habitat.

In some mammals that breed cooperatively, nonbreeding female helpers nurse offspring born to the breeding female in their group, a process known as allonursing. Previous laboratory studies have suggested that primiparous and multiparous females (those that have successfully given birth once or more, respectively) are more likely to contribute to allonursing than nulliparous females (those that have not given birth). In contrast, few field studies have been able to demonstrate whether breeding experience, or parity, affects the likelihood or duration of allonursing. In this study, we investigated whether the likelihood and duration of allonursing by subordinate female meerkats (Suricata suricatta) were associated with variations in their parity (number of pregnancies reaching a viable gestational age) and their immediate pregnancy status. Comparisons showed that nonpregnant nulliparous females were less likely to allonurse than nonpregnant primiparous and multiparous females, although parity was not related to variation in allonursing duration. Parity may enhance the expression of maternal behavior when exposed to foster pups, especially since mammary gland development has been shown to be directly associated with parity in other mammals.

Mammals typically process food items more extensively in their oral cavities than do other vertebrates. Dental morphology, jaw-muscle activity patterns, mandibular movements, and tongue manipulation work to facilitate oral fragmentation of dietary items. While processing mechanically challenging foods, mammals modulate mandibular movements and bite forces via recruitment of greater jaw-adductor muscle forces and protracted biting or chewing. Because jaw-loading patterns are influenced by magnitude; frequency; and duration of muscular, bite, and reaction forces during routine feeding behaviors, relatively larger jaws are thought to be more characteristic of mammals that experience higher masticatory loads due to the processing of mechanically challenging foods. The ease of food fracture during post-canine biting and chewing is mainly determined by food stiffness and toughness. Such foods have been associated with increased loading magnitude and/or greater amounts of cyclical loading (i.e., chewing duration). Dietary properties are thought to modulate cyclical loading through changes in chewing frequency and chewing investment. On the other hand, chewing frequency has been found to be independent of dietary properties in rabbits and primates; however, little evidence exists regarding the influence of dietary properties on these parameters in a broader range of mammals. Here, we assessed chewing behavior in seven adult llamas (Llama glama) processing foods with a wide range of mechanical properties (grain, hay, carrots, and dried corn). Each subject was filmed at 60 frames/s, with video slowed for frame-by-frame computer analysis to obtain length of feeding bout and number of chewing cycles for each food type. These parameters were used to calculate chewing frequency (chews/s), chewing investment (chews/g), and chewing duration (s/g). Chewing frequency was not significantly related to mechanical properties of food, but chewing investment and chewing duration were significantly related to dietary stiffness and toughness. Therefore, cyclical loading is positively influenced by stiff and tough foods. This suggests that variation in jaw morphology in extinct and extant mammals is positively related to dietary stiffness and toughness, which requires greater chewing investment and increased chewing duration.

Atlantic Forest (AF) is amongst the most threatened forests in the world. To decide where conservation efforts should be focused to preserve species, assessment of ecological and biogeographic processes nowadays are crucial. Patterns of the distribution of organisms can provide an important source of information underlying the biogeographical history of a biota. Here, our main objective was to identify Areas of Endemism (AoE) for non-volant small mammals in the AF and to investigate if those AoE are covered by protected areas. We performed quantitative and qualitative approaches to delimit AoE and calculated the area overlaid by Conservation Units (CU) within each AoE. Our results supported the recognition of seven AoE for small mammals in the AF, which largely are congruent with previous studies undertaken on other organisms, thereby highlighting the importance of those regions as hotspots of endemism. Most of the AoE recovered in the present study have less than 12% of their territory covered by forest remnants, and less than 11% of their entire range is under legal protection. These findings bring to light an important discussion on how information pertaining to the representativeness of CU within regions of high endemicity could help to identify areas in need of urgent protection within a threatened biodiversity hotspot.

In the Andean portion of northern Patagonia, populations of Ctenomys are found from low-elevation plains to high-elevation meadows and valleys. Despite their prevalence, the taxonomy of these subterranean rodents remains poorly resolved. Using genetic and morphological data obtained from museum specimens and animals collected in the field, we examined the taxonomy of Ctenomys from southwestern Mendoza Province, Argentina. Our analyses suggest the presence of at least five species of Ctenomys within the study area. The highest, innermost portion of the Andes is occupied by C. maulinus. To the east, the mountains and foothills are inhabited by two forms associated with the “mendocinus” and the “magellanicus” lineages, respectively. The former appears to be a local variant of C. emilianus, while the latter is sister to C. pontifex. Although C. pontifex was not encountered during our field sampling, it remains a valid species that likely is restricted to the isolated Valle Hermoso in westernmost Mendoza Province. In addition, we report an undescribed form from Las Leñas Valley that is associated with the “mendocinus” lineage. This complex alpha taxonomic scenario occurs within less than one degree of latitude, thereby highlighting the need to conduct detailed field collections to improve our knowledge of the systematics of Ctenomys.

Fourteen species of bats in the genus Molossus currently are recognized in the Neotropical region; only three are known from Argentina. Here, we describe a new species based on specimens collected in the province of Santa Fe, Argentina, in the Pampa ecoregion. The new species can be distinguished from its congeners by its general strong orange coloration, forearm length > 41 mm, dorsal hairs bicolor and long (∼5 mm), infraorbital foramen laterally oriented, and long and forward-projected (pincer-like) upper incisors. The external and cranial morphology of the new species are described and comparisons made with other species of similar size and with those present in its distributional area. Wilcoxon tests and multivariate analyses (nonmetric multidimensional scaling ordination and PERMANOVA) were carried out to determine the morphometric differences between the new species and other seven species of Molossus. The species tree, estimated by *BEAST from the concatenation of mitochondrial and nuclear genes, suggests that Molossus sp. nov. is basal within the clade formed by {{M. aztecus, M. rufus}, {{M. currentium, M. pretiosus}, M. sinaloae}} with a posterior probability of 0.82.