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1 January 2004 Extracts of Scabies Mites (Sarcoptidae: Sarcoptes scabiei) Modulate Cytokine Expression by Human Peripheral Blood Mononuclear Cells and Dendritic Cells
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Abstract

We performed a series of experiments to determine if human peripheral blood mononuclear cells (PBMCs) from a healthy donor and dendritic cells (NHDCs) derived from these PBMCs reacted to molecules in a scabies extract. PBMCs extravasate from the circulatory system and enter tissues such as scabietic lesions, where monocytes become macrophages. Cells were cultured in medium alone or medium containing 50 μg/ml of Sarcoptes scabiei (SS) extract, 50 ng/ml E. coli lipopolysaccharide (LPS), or SS LPS together. Supernatants were collected and assayed by enzyme-linked immunosorbent assay (ELISA) for specific cytokines. PBMCs stimulated with SS or LPS exhibited moderately upregulated production of interleukin (IL)-1β and huge increases in secretions of IL-6, IL-8 and TNF-α. Cells co-stimulated with both SS and LPS generally secreted more of these cytokines than cells stimulated with either SS or LPS alone. LPS induced a small amount of IL-1α secretion, whereas SS did not, and neither additive resulted in the production of IL-10. NHDCs did not produce IL-1α, IL-1β, IL-6, IL-8, or IL-10 in response to stimulation with SS. These cells did produce IL-6, IL-8, and tumor necrosis factor (TNF)-α in response to LPS. When cells were co-stimulated with both LPS and SS, the production of IL-6 and IL-8 was significantly reduced compared with the levels secreted after LPS stimulation alone. These studies show that molecules in a whole body extract of S. scabiei modulate the function of PBMCs (probably monocytes) and dendritic cells.

Larry G. Arlian, Marjorie S. Morgan, and Jacqueline S. Neal "Extracts of Scabies Mites (Sarcoptidae: Sarcoptes scabiei) Modulate Cytokine Expression by Human Peripheral Blood Mononuclear Cells and Dendritic Cells," Journal of Medical Entomology 41(1), 69-73, (1 January 2004). https://doi.org/10.1603/0022-2585-41.1.69
Received: 27 May 2003; Accepted: 1 August 2003; Published: 1 January 2004
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