Translator Disclaimer
1 July 2005 The Hyrcanus Group of Anopheles (Anopheles) in China (Diptera: Culicidae): Species Discrimination and Phylogenetic Relationships Inferred by Ribosomal DNA Internal Transcribed Spacer 2 Sequences
Author Affiliations +
Abstract

The Hyrcanus group comprises many closely related species with wide distributions in the Oriental and Palaearctic regions. The sequences of the second internal transcribed spacer (ITS2) of ribosomal DNA were determined for 12 species in China—An. crawfordi, An. hyrcanus, An. junlianensis, An. kunmingensis, An. kweiyangensis, An. lesteri, An. liangshanensis, An. peditaeniatus, An. pullus, An. sinensis, and two unknown species within the group. The length of the ITS2 ranged from 436 bp in An. hyrcanus to 469 bp in An. crawfordi, with GC contents of 44.9–46.8%. Intraspecific variation was found in three species (An. junlianensis, An. liangshanensis, and An. pullus) at the level of 0.0–0.4%, whereas interspecific differences ranged from 1.6% between An. liangshanensis and An. kunmingensis to 50.8% between An. peditaeniatus and sp. 1. The ITS2 comparisons revealed two unknown species, verified the valid species status for An. kunmingensis, and found An. pullus in China. We agree that An. anthropophagus is a junior synonym of An. lesteri. The validation of An. junlianensis awaits recognition of the molecular identity of the entity identified as An. yatsushiroensis. The ITS2 divergences were used for inferring phylogenetic relationships among 12 species in China. The estimation revealed close relationships among An. liangshanesis, An. kunmingensis, An. kweiyangensis, An. lesteri, and An. sinensis. Our study emphasizes the need for the molecular identity of the species members in integrated studies in systematics, bionomics, and population genetics for the Hyrcanus group.

Yajun Ma and Jiannong Xu "The Hyrcanus Group of Anopheles (Anopheles) in China (Diptera: Culicidae): Species Discrimination and Phylogenetic Relationships Inferred by Ribosomal DNA Internal Transcribed Spacer 2 Sequences," Journal of Medical Entomology 42(4), 610-619, (1 July 2005). https://doi.org/10.1603/0022-2585(2005)042[0610:THGOAA]2.0.CO;2
Received: 16 September 2003; Accepted: 11 February 2005; Published: 1 July 2005
JOURNAL ARTICLE
10 PAGES

This article is only available to subscribers.
It is not available for individual sale.
+ SAVE TO MY LIBRARY

SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top