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1 July 2005 Detection of Babesia microti DNA in Ixodes scapularis (Acari: Ixodidae) by Use of Chelex 100 Resin and Polymerase Chain Reaction
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Abstract

A rapid and sensitive protocol using Chelex 100 resin for DNA extraction and the polymerase chain reaction for detection of Babesia microti Franca within its vector Ixodes scapularis Say is described. The level of detection of this protocol was determined to be the approximate equivalent of one parasite.

Charles Thill, P. Bryon Backenson, Melissa A. Prusinski, Sarah J. Kogut, Joon-Hak Lee, and James L. Coleman "Detection of Babesia microti DNA in Ixodes scapularis (Acari: Ixodidae) by Use of Chelex 100 Resin and Polymerase Chain Reaction," Journal of Medical Entomology 42(4), 694-696, (1 July 2005). https://doi.org/10.1603/0022-2585(2005)042[0694:DOBMDI]2.0.CO;2
Received: 4 October 2004; Accepted: 2 March 2005; Published: 1 July 2005
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