Translator Disclaimer
1 May 2006 Sequence Analysis of the rDNA Internal Transcribed Spacer 2 and Polymerase Chain Reaction Identification of Anopheles fluminensis (Diptera: Culicidae: Anopheles) in Bolivia
Author Affiliations +
Abstract

Anopheles fluminensis Root is a member of the Arribalzagia Series in the subgenus Anopheles. We report the first record of this species in the department of Cochabamba, Bolivia. This species was sampled from two locations in the foothills of the eastern Andes Mountains within the Chapare Valley. Larvae were collected in fast-flowing, shaded streams at the edges of rocky pools. We provide the first sequence data for the rDNA of An. fluminensis, a partial sequence of the 5.8S and the internal transcribed spacer 2 (ITS2). The ITS2 of An. fluminensis, sequenced from two individuals at one site, was at least 596 bp, had 56.5% GC, and included three large repeats (≈125 bp each). We describe a polymerase chain reaction protocol and species-specific primers for identifying this species in the Chapare Valley, Bolivia.

COREY L. BRELSFOARD, GARY N. FRITZ, and ROBERTO RODRIGUEZ "Sequence Analysis of the rDNA Internal Transcribed Spacer 2 and Polymerase Chain Reaction Identification of Anopheles fluminensis (Diptera: Culicidae: Anopheles) in Bolivia," Journal of Medical Entomology 43(3), 460-466, (1 May 2006). https://doi.org/10.1603/0022-2585(2006)43[460:SAOTRI]2.0.CO;2
Received: 2 August 2005; Accepted: 29 December 2005; Published: 1 May 2006
JOURNAL ARTICLE
7 PAGES

This article is only available to subscribers.
It is not available for individual sale.
+ SAVE TO MY LIBRARY

SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top