16S rRNA Gene Sequences from Bacteria Associated with Adult Anopheles darlingi (Diptera: Culicidae) Mosquitoes

Olle Terenius; Caroline Dantas de Oliveira; Waleria Dasso Pinheiro; Wanderli Pedro Tadei; Anthony Amade James; Osvaldo Marinotti

doi:10.1603/0022-2585(2008)45[172:SRGSFB]2.0.CO;2

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1 January 2008 16S rRNA Gene Sequences from Bacteria Associated with Adult Anopheles darlingi (Diptera: Culicidae) Mosquitoes

Olle Terenius, Caroline Dantas de Oliveira, Waleria Dasso Pinheiro, Wanderli Pedro Tadei, Anthony Amade James, Osvaldo Marinotti

Author Affiliations +

Olle Terenius,^* Caroline Dantas de Oliveira,^** Waleria Dasso Pinheiro,^** Wanderli Pedro Tadei,^** Anthony Amade James,^*,*** Osvaldo Marinotti^*,****

^*Department of Molecular Biology and Biochemistry, 3205 McGaugh Hall, University of California, Irvine, CA 92697.
^**Instituto Nacional de Pesquisas da Amazonia, Manaus, AM 69011-970, Brazil.
^***Department of Microbiology and Molecular Genetics, University of California, Irvine, CA 92697.
^****Corresponding author, e-mail: omarinot@uci.edu.

J. of Medical Entomology, 45(1):172-175 (2008). https://doi.org/10.1603/0022-2585(2008)45[172:SRGSFB]2.0.CO;2

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Abstract

The microbial flora associated with Anopheles darlingi Root (Diptera: Culicidae), a major Neotropical malaria vector, was investigated for the development of a paratransgenesis-based approach to control malaria transmission in Brazil. Female mosquitoes were collected using human land catches and captured insects provided a bloodmeal. The controlled blood feeding resulted in increased detection of mosquito bacterial population because it was possible to retrieve bacterial DNA from all blood-fed mosquitoes. The 16S sequences of bacteria recovered, include some closely related to those found in other vector mosquitoes, including Aeromonas, Pantoea and Pseudomonas species.

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Olle Terenius, Caroline Dantas de Oliveira, Waleria Dasso Pinheiro, Wanderli Pedro Tadei, Anthony Amade James, and Osvaldo Marinotti "16S rRNA Gene Sequences from Bacteria Associated with Adult Anopheles darlingi (Diptera: Culicidae) Mosquitoes," Journal of Medical Entomology 45(1), 172-175, (1 January 2008). https://doi.org/10.1603/0022-2585(2008)45[172:SRGSFB]2.0.CO;2

Received: 24 April 2007; Accepted: 11 September 2007; Published: 1 January 2008

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