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1 March 2009 Molecular Genetic Investigation of Morphological Species of Members of the Neomelaniconion Subgenus (Diptera: Culicidae: Aedini) From Madagascar Using Ribosomal Internal Transcribed Spacer 2
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Abstract

Species of Neomelaniconion (Neo.) are aggressive toward humans and domestic animals. Some of these species play a role in the transmission of arbovirus. In Madagascar, separation of these arbovirus vectors was based on doubtful morphological features. Sequence variation of the ribosomal DNA internal transcribed spacer 2 from morphological Neomelaniconion species of the Neomelaniconion subgenus from Madagascar was investigated to assess the validity of morphologically defined taxa. Both methods showed two taxonomic groups: the Circumluteolus Group that includes Aedes (Neo.) belleci (Le Goff, Bousses, and Brunhes, 2007), Ae. (Neo.) nigropterum (Le Goff, Bousses, and Brunhes, 2007), and Ae. (Neo.) circumluteolus (Theobald, 1908); and the Sylvaticum Group that includes Ae. (Neo.) sylvaticum (Le Goff, Bousses,and Brunhes, 2007), Ae. (Neo.) fontenillei (Le Goff, Bousses, and Brunhes, 2007), and Ae. (Neo.) albiradius (Le Goff, Bousses, and Brunhes, 2007). Although sequence analysis failed to completely resolve each group, four distinct patterns of sequence variation supported by several fixed mutations were shown from specimens in Madagascar.

© 2009 Entomological Society of America
Pierre Kengne, Gilbert Le Goff, and Didier Fontenille "Molecular Genetic Investigation of Morphological Species of Members of the Neomelaniconion Subgenus (Diptera: Culicidae: Aedini) From Madagascar Using Ribosomal Internal Transcribed Spacer 2," Journal of Medical Entomology 46(2), 403-407, (1 March 2009). https://doi.org/10.1603/033.046.0230
Received: 30 June 2008; Accepted: 1 December 2008; Published: 1 March 2009
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