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1 September 2010 Cloning, Expression, and Characterization of Der f 7, an Allergen of Dermatophagoides farinae from China
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A full-length cDNA encoding house dust mite allergen Der f 7 from Dermatophagoides farina. (Acari: Pyroglyphidae) from China was cloned, sequenced, and successfully expressed. A reference sequence (GenBank accession AY283292) was used to design polymerase chain reaction primers. Analysis revealed eight mismatched nucleotides in five Der f 7 cDNA clones, and the projected amino acid sequence contained six incompatible residues. These results suggest that the sequence of Der f 7 may be polymorphic. Further bioinformatic analysis revealed that the mature Der f 7 allergen had a molecular mass of ≈21.88 kDa and a theoretical isoelectric point of 4.90. Der f 7 protein secondary structure was composed of α helix (56.63%), extended strand (5.10%), and random coil (38.27%). Group 7 allergens are present in Pyroglyphidae, Acaridae, and Glycyphagidae families, and homology analysis revealed a 86% similarity between Der f 7 and Der p 7. Furthermore, a phylogenetic tree constructed of group 7 allergens from different mite species revealed that Der f 7 and Der p 7 clustered with 100% bootstrap support. Bioinformatics-driven characterization of Der f 7 allergen as conducted in this study may contribute to diagnostic and therapeutic applications for dust mite allergies.

© 2010 Entomological Society of America
Yu-Bao Cui, Hong-Xing Cai, Ying Zhou, Cui-Xiang Gao, Wei-Hong Shi, Ming Yu, and Li Li "Cloning, Expression, and Characterization of Der f 7, an Allergen of Dermatophagoides farinae from China," Journal of Medical Entomology 47(5), 868-876, (1 September 2010).
Received: 10 July 2009; Accepted: 1 May 2010; Published: 1 September 2010

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