Species-Diagnostic Polymerase Chain Reaction Assays for Phlebotomus argentipes and Phlebotomus papatasi, Vectors of Leishmania

A. M. Manonmani; A. Mathivanan; R. Srinivasan; P. Jambulingam

doi:10.1603/ME09195

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1 September 2010 Species-Diagnostic Polymerase Chain Reaction Assays for Phlebotomus argentipes and Phlebotomus papatasi, Vectors of Leishmania

A. M. Manonmani, A. Mathivanan, R. Srinivasan, P. Jambulingam

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J. of Medical Entomology, 47(5):743-747 (2010). https://doi.org/10.1603/ME09195

Abstract

Species-specific differences encountered in the nucleotide sequences of a highly variable region of the 18S rRNA gene were used to design a multiplex polymerase chain reaction (PCR) assay for the identification of Phlebotomus papatasi Scopoli and Phlebotomus argentipes Annandale & Brunetti, vectors of Leishmania. This multiplex PCR assay uses a common forward primer and two reverse primers, which are specific for the two species. Amplification of a PCR product of size 788 bp indicates the presence of P. papatasi, whereas a product of size 677 bp indicates the presence of P. argentipes. The assay was found to be highly specific and sensitive.

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A. M. Manonmani, A. Mathivanan, R. Srinivasan, and P. Jambulingam "Species-Diagnostic Polymerase Chain Reaction Assays for Phlebotomus argentipes and Phlebotomus papatasi, Vectors of Leishmania," Journal of Medical Entomology 47(5), 743-747, (1 September 2010). https://doi.org/10.1603/ME09195

Received: 27 July 2009; Accepted: 1 April 2010; Published: 1 September 2010

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