Species-specific differences encountered in the nucleotide sequences of a highly variable region of the 18S rRNA gene were used to design a multiplex polymerase chain reaction (PCR) assay for the identification of Phlebotomus papatasi Scopoli and Phlebotomus argentipes Annandale & Brunetti, vectors of Leishmania. This multiplex PCR assay uses a common forward primer and two reverse primers, which are specific for the two species. Amplification of a PCR product of size 788 bp indicates the presence of P. papatasi, whereas a product of size 677 bp indicates the presence of P. argentipes. The assay was found to be highly specific and sensitive.
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1 September 2010
Species-Diagnostic Polymerase Chain Reaction Assays for Phlebotomus argentipes and Phlebotomus papatasi, Vectors of Leishmania
A. M. Manonmani,
A. Mathivanan,
R. Srinivasan,
P. Jambulingam
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Journal of Medical Entomology
Vol. 47 • No. 5
September 2010
Vol. 47 • No. 5
September 2010
PCR assay
Phlebotomus argentipes
Phlebotomus papatasi