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1 September 2014 Molecular Detection of Knockdown Resistance (kdr) in Blattella germanica (Blattodea: Blattellidae) From Northwestern Iran
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Abstract

Pyrethroid insecticides are highly insecticidal compounds that are widely used against the German cockroach, a significant household insect pest. In several insect species, there is a point mutation in the para-type sodium channel gene associated with knockdown resistance (kdr). In the current study, genomic DNA was analyzed in the region where the kdr and super-kdr (an enhanced form of pyrethroid resistance) mutations reside in Blatella germanica (L., 1767) (Blattodea: Blattellidae) collected from Iran. Studies on the extracted DNA from hand-captured German cockroach specimens were conducted by polymerase chain reaction and sequencing to detect related mutations. The kdr mutation, substitution of G for C (L1014F), which results in amino acid replacement (leucine with phenylalanine), was detected in all 18 sequenced specimens from three different locations. However, the super-kdr mutation (M918T), which is detected in super-kdr house flies, was not found in the sequences of the current study. The high ratio of the kdr mutation in a field population of B. germanica in Urmia confirms that the individuals are homozygous. These data should be helpful in designing and implementing a control program and resistance management.

© 2014 Entomological Society of America
S. Gholizadeh, B. Nouroozi, and H. Ladonni "Molecular Detection of Knockdown Resistance (kdr) in Blattella germanica (Blattodea: Blattellidae) From Northwestern Iran," Journal of Medical Entomology 51(5), 976-979, (1 September 2014). https://doi.org/10.1603/ME13160
Received: 11 August 2013; Accepted: 1 June 2014; Published: 1 September 2014
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