Translator Disclaimer
1 November 2014 Novel Primers from Informative Nuclear Loci for Louse Molecular Phylogenetics (Insecta: Phthiraptera)
Author Affiliations +
Abstract

While parasitic lice (Insecta: Phthiraptera) have historically been an important model taxon for understanding host-parasite coevolution, very few molecular markers have been developed for phylogenetic analysis. The current markers are insufficient to resolve many of the deeper nodes in this group; therefore, sequences from additional genetic loci are necessary. Here, we design primers targeting several nuclear protein coding genes based on a complete genome and transcriptome of Pediculus humanus L. plus transcriptomes and modest coverage genomic data from five genera of avian feather lice. These primers were tested on 32 genera of avian feather lice (Ischnocera), including multiple species within some genera. All of the new primer combinations produced sequences for the majority of the genera and had similar or higher divergences than the most widely used nuclear protein-coding gene in lice, EF-1α. These results indicate that these new loci will be useful in resolving phylogenetic relationships among parasitic lice.

© 2014 Entomological Society of America
Andrew D. Sweet, Julie M. Allen, and Kevin P. Johnson "Novel Primers from Informative Nuclear Loci for Louse Molecular Phylogenetics (Insecta: Phthiraptera)," Journal of Medical Entomology 51(6), 1122-1126, (1 November 2014). https://doi.org/10.1603/ME13218
Received: 7 November 2013; Accepted: 1 August 2014; Published: 1 November 2014
JOURNAL ARTICLE
5 PAGES

This article is only available to subscribers.
It is not available for individual sale.
+ SAVE TO MY LIBRARY

SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top