Previous published evidence for the occurrence of an exotic armadillo tick, Amblyomma auricularium (Conil), in Florida is scant, but we found it is fully established and integrated into the state's tick fauna. We collected 11,192 specimens of this tick from naturalized nine-banded armadillos, Dasypus novemcinctus L., and 14 other species of wild native mammals and birds in Florida, while sampling statewide during 2004 through 2007. In all, we document its specific presence only in 14 contiguous South Florida counties. Moreover, we report the first collections of A. auricularium from the Virginia opossum (Didelphis virginiana Kerr), common raccoon [Procyon lotor (L.)], cotton deermouse [Peromyscus gossypinus (Le Conte)], gray fox [Urocyon cinereoargenteus (Schreber)], eastern spotted skunk [Spilogale putorius (L.)], and white-tailed deer [Odocoileus virginianus (Zimmerman)]. For the first time on birds, we report the collection of this tick from the broad-winged hawk [Buteo platypterus (Vieillot)], northern cardinal [Cardinalis cardinalis (L.)], Carolina wren [Thryothorus ludovicianus (Latham)], gray catbird [Dumetella carolinensis (L.)], and yellow-rumped warbler [Setophaga coronata (L.)]. In addition, we report unattached A. auricularium collected from humans for the first time, and additional new collections from domestic dogs, Canis lupus familiaris L.