Triatominae bugs (Hemiptera: Reduviidae) are usually associated with different vertebrate species, upon which many of them feed. Yet how these different blood meal sources influence key biological parameters is rarely investigated for triatomines. To fill this knowledge gap, this study sought to determine the effect of a domestic rat species (Rattus norvegicus Berkenhout (Rodentia: Muridae)), a domestic mice species (Mus musculus L. (Rodentia: Muridae)), and chickens (Gallus gallus domesticus L. (Galliformes: Phasianidae)), as blood meal sources upon several biological parameters (development time, number of required blood meals to moult and feeding and defecation behaviors) of the Mexican major vector Triatoma barberi Usinger. The three studied cohorts' development times were similar (325–338 d), but the number of required blood meals to moult (21), as well as the total mortality rate (26%), were both the highest in the cohort that fed on chickens. The longevity of females (186–190 d) was similar among the three studied cohorts, as was that of males. The median time elapsed between the presentation of a blood meal source and onset of feeding (10 min) was similar among the three studied cohorts, as were their feeding times and defecation patterns. Most of our studied parameters demonstrate how T. barberi can effectively take advantage of feeding on rodents as much as it does on hens. Those parameter results also show that T. barberi should be considered as a potential yet underappreciated vector in some areas, thus warranting a surveillance program of its current distribution area in Mexico.