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3 May 2023 Development of an automated mosquito pupae counter
Jérémy Esnault, Sarah Scussel, Benjamin Gaudillat, Quentin Lejarre, Matéo Toussaint, Marianne Duployer, Miklós Gárdos, Péter Bán, Patrick Mavingui, Pablo Tortosa, Julien Cattel
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Abstract

The production of mosquitoes for control programs or basic research is facilitated by the standardization of rearing conditions allowing the daily manipulation of thousands of individuals. It is crucial to develop mechanical or electronic systems for controlling the density of mosquitoes at each development stage with the aim of reducing costs, time, and human errors. We present herein an automatic mosquito counter using a recirculating water system allowing rapid and reliable counting of pupae without detectable increased mortality. Using Aedes albopictus pupae, we determined the density of pupae and the time of counting for which the device is most accurate, and evaluated the time saved using this device. Lastly, we discuss how this mosquito pupae counter can be useful in small-scale or mass-rearing contexts enabling a number of applications for research purposes as well as operational mosquito control programs.

Jérémy Esnault, Sarah Scussel, Benjamin Gaudillat, Quentin Lejarre, Matéo Toussaint, Marianne Duployer, Miklós Gárdos, Péter Bán, Patrick Mavingui, Pablo Tortosa, and Julien Cattel "Development of an automated mosquito pupae counter," Journal of Medical Entomology 60(4), 828-832, (3 May 2023). https://doi.org/10.1093/jme/tjad050
Received: 30 January 2023; Accepted: 19 April 2023; Published: 3 May 2023
JOURNAL ARTICLE
5 PAGES

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KEYWORDS
Aedes albopictus
automation
disease vectors
male-sterile based strategy
mosquito control
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