A Novel, Simplified Technique to Amplify Eimeria (Coccidia: Apicomplexa) DNA from Oocysts

R. W. Gerhold; L. R. McDougald; R. B. Beckstead

doi:10.1645/14-524.1

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R. W. Gerhold,¹ L. R. McDougald,¹ R. B. Beckstead¹

¹Department of Poultry Science, University of Georgia, Athens, Georgia 30602;

Abstract

A new method to amplify coccidia DNA by polymerase chain reaction (PCR) was developed by placing freeze-thawed oocysts in Ready-to-Go PCR bead tubes and using a 5-min initial heat denaturation step. Positive PCR reactions were found in 3 of 3 samples containing 20 or 50 oocysts; when ≤5 oocysts were used, 1 of 3 samples was positive. This technique shows potential for effectively and efficiently detecting and identifying oocysts from soil, feces, and other matter.

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R. W. Gerhold, L. R. McDougald, and R. B. Beckstead "A Novel, Simplified Technique to Amplify Eimeria (Coccidia: Apicomplexa) DNA from Oocysts," Journal of Parasitology 101(1), 102-103, (1 February 2015). https://doi.org/10.1645/14-524.1

Published: 1 February 2015

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