As a part of an ongoing project to inventory the helminth parasites of rodents in Mexico, 85 specimens of 2 families of rodents were collected from the Mexican Plateau: Cricetidae (Neotoma sp., Neotoma leucodon, Onychomys arenicola, Peromyscus sp., Peromyscus eremicus, and Reithrodontomys sp.) and Heteromyidae (Chaetodipus sp., Chaetodipus eremicus, Chaetodipus hispidus, Dipodomys merriami, Dipodomys ordii, Dipodomys ornatus, Dipodomys spectabilis, Liomys irroratus, Perognathus sp., and Perognathus flavus). A total of 13 taxa of helminths were found: Heteromyoxyuris longejector, Heteromyoxyuris otomii, Heteromyoxyuris sp., Onchocercidae gen. sp. 1 and sp. 2, Physalopteridae gen. sp., Protospirura dipodomis, Pterygodermatites dipodomis, Subulura sp., Syphacia sp., Trichuris dipodomis, Vexillata liomyos, and Vexillata armande. The highest species richness was recorded in D. merriami (7 taxa). This study is the first report of nematodes from O. arenicola (Physalopteridae gen. sp.) and C. eremicus (H. longejector) and for V. liomyos from D. merriami. All reports of these species of nematodes represent new collection localities in Mexico.