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1 December 2020 A Case-Study of the Molecular Diagnosis of Echinococcus multilocularis in Wild Boar with Comments on Its Public Health Significance in Turkey
Harun Kaya Kesik, Seyma Gunyakti Kilinc, Figen Celik, Sami Simsek, Haroon Ahmed
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Abstract

Echinococcus multilocularis is a parasite species of zoonotic importance which can be fatal to humans and causes Alveolar Echinococcosis (AE). This report describes the development of a cyst from the liver of a wild boar and molecular confirmation of its identification. The cyst material was obtained from the liver of a wild boar killed by hunters. Genomic DNA was extracted from the germinal layer of the cyst material, and 875 bp mitochondrial cytochrome c oxidase subunit I (COI) gene fragment was amplified by PCR and sequenced. A BLAST search matched 100% with published Echinococcus multilocularis sequences. This study confirms the occurrence of E. multilocularis in a wild boar for the first time in Turkey.

© American Society of Parasitologists 2020
Harun Kaya Kesik, Seyma Gunyakti Kilinc, Figen Celik, Sami Simsek, and Haroon Ahmed "A Case-Study of the Molecular Diagnosis of Echinococcus multilocularis in Wild Boar with Comments on Its Public Health Significance in Turkey," Journal of Parasitology 106(6), 730-734, (1 December 2020). https://doi.org/10.1645/19-196
Published: 1 December 2020
JOURNAL ARTICLE
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KEYWORDS
Echinococcus multilocularis
PCR
sequencing
Turkey
wild boar
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