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7 January 2022 Giardia duodenalis Genotyping Not Linked to Clinical Symptomatology and Nutritional Status of School-Aged Children of Soledad and Galapa Municipality Schools, Atlántico, Colombia
Karen Muñoz Salas, Alma Polo Barrios, Carolina Maestre Gonzalez, Juan Rodríguez Macias, Carmiña Vargas Zapata
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Abstract

Giardia duodenalis genotypes A and B have been reported in Colombia. The population consisted of 235 schoolchildren whose ages ranged from 2 to 10 yr of age from the municipalities of Soledad and Galapa in the department of Atlántico, Colombia. Fecal samples were obtained and then analyzed in triplicate using the sedimentation in formalin-ether (Ritchie's method) and direct examination techniques. Of the 235 fecal samples, 35 samples were positive for G. duodenalis; positive samples were concentrated in a sucrose gradient and sonicated for 3 cycles of 20 sec. DNA extraction was performed, and the parasites were genotyped by conventional PCR amplifying a region of the β-giardin gene. A general prevalence of G. duodenalis of 13.2% was found, and of these genotyped samples, 13 (56.7%) and 7 (20%) corresponded to genotype A, 1 (4.3%), and 3 (25%) corresponded to genotype B, and 9 (39.1%) and 2 (16.7%) were not defined, in the municipalities Soledad and Galapa, respectively. Additionally, 23 children were diagnosed with symptomatologic giardiasis, and 12 were asymptomatic; the most relevant symptoms were abdominal pain (7, 20%) and diarrhea (13, 56.7%). The nutritional status of children with Giardia genotypes A and B were as follows: 3 in a state of malnutrition (10%), 10 normal (33.3%), and 6 overweight and obese (20%) with genotype A, and 1 in a state of malnutrition (3.3%) and 3 normal (10%) with genotype B. The genotypes found in G. duodenalis did not show an association with nutritional status or with the clinical manifestations evaluated in schoolchildren.

© American Society of Parasitologists 2022
Karen Muñoz Salas, Alma Polo Barrios, Carolina Maestre Gonzalez, Juan Rodríguez Macias, and Carmiña Vargas Zapata "Giardia duodenalis Genotyping Not Linked to Clinical Symptomatology and Nutritional Status of School-Aged Children of Soledad and Galapa Municipality Schools, Atlántico, Colombia," Journal of Parasitology 108(1), 1-9, (7 January 2022). https://doi.org/10.1645/21-26
Published: 7 January 2022
JOURNAL ARTICLE
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KEYWORDS
children
clinical manifestations
genotypes
Giardia duodenalis
giardiasis
malnutrition
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