28 December 2022 Morphological and Genetic Characterization of Didelphonema longispiculata (Nematoda: Spiruroidea) in the Black-Eared Opossum Didelphis marsupialis
Leodil da Costa Freitas, Beatriz Elise de Andrade Silva, Roberto do Val Vilela, Ravena Fernanda Braga de Mendonça, Rogério Vieira Rossi, Arnaldo Maldonado Júnior, Richard de Campos Pacheco
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Abstract

Didelphonema longispiculata (Hill, 1939), a gastric nematode parasite of the black-eared opossum, Didelphis marsupialis Linnaeus, 1758, collected from 2 municipalities of Mato Grosso state, Brazil, in the ecotone region of the Amazon and Cerrado biomes was analyzed with integrative taxonomy using light and scanning electron microscopy (SEM) for morphological studies and sequencing of the 18S small subunit ribosomal RNA for phylogenetic inference through maximum likelihood and Bayesian phylogenetic inference. Here details of the helminth surface, oral aperture with octagonal border, pseudo- and inter-labia, amphids, external cephalic papillae, 2 dorsal and ventral internal plates distally indented, and stoma with strongly chitinized wall are presented. Caudal male papillae, spicules, female vulva, anus, and caudal tip were detailed using SEM. Morphological characteristics and phylogenetic data corroborated the taxonomic placement of the genus Didelphonema within the subfamily Ascaropsinae.

© American Society of Parasitologists 2022
Leodil da Costa Freitas, Beatriz Elise de Andrade Silva, Roberto do Val Vilela, Ravena Fernanda Braga de Mendonça, Rogério Vieira Rossi, Arnaldo Maldonado Júnior, and Richard de Campos Pacheco "Morphological and Genetic Characterization of Didelphonema longispiculata (Nematoda: Spiruroidea) in the Black-Eared Opossum Didelphis marsupialis," Journal of Parasitology 108(6), 627-636, (28 December 2022). https://doi.org/10.1645/21-59
Published: 28 December 2022
KEYWORDS
Ascaropsinae
Didelphis marsupialis
Didelphonema longispiculata
Nematoda
neotropical
phylogeny
Southeastern Amazon
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