Molecular Differentiation of Three Species of Metagonimus by Simple Sequence Repeat Anchored Polymerase Chain Reaction (SSR-PCR) Amplification

H-J. Yang; S-M. Guk; E-T. Han; J-Y. Chai

doi:10.1645/0022-3395(2000)086[1170:MDOTSO]2.0.CO;2

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H-J. Yang,¹ S-M. Guk,² E-T. Han,³ J-Y. Chai^3,4

¹Department of Parasitology, Cheju National University College of Medicine, Cheju 690-756, Korea
²Department of Parasitology, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center, Seoul 110-799, Korea
³Department of Parasitology, Yonsei University College of Medicine, Seoul 120-752, Korea
⁴author for correspondence

Abstract

Genomic DNAs from 3 species of Metagonimus in the Republic of Korea, M. yokogawai, M. miyatai, and M. takahashii, were amplified by simple sequence repeat anchored polymerase chain reaction. Primers of 3′ or 5′ termini of the (CA)_n repeats were used; 3 kinds of 3′ anchored primers (CA)₈RG, (CA)₄RG, and (CA)₈RY, and 2 kinds of 5′ anchored primers BDB(CA)₇C and DBDA(CA)₇. The results showed distinguishable banding patterns among the 3 species of Metagonimus, suggesting that they have different genotypes.

Citation Download Citation

H-J. Yang, S-M. Guk, E-T. Han, and J-Y. Chai "Molecular Differentiation of Three Species of Metagonimus by Simple Sequence Repeat Anchored Polymerase Chain Reaction (SSR-PCR) Amplification," Journal of Parasitology 86(5), 1170-1172, (1 October 2000). https://doi.org/10.1645/0022-3395(2000)086[1170:MDOTSO]2.0.CO;2

Published: 1 October 2000

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