Isolation of Neospora caninum From the Brain of a Pregnant Sheep

T. Koyama; Y. Kobayashi; Y. Omata; M. Yamada; H. Furuoka; R. Maeda; T. Matsui; A. Saito; T. Mikami

doi:10.1645/0022-3395(2001)087[1486:IONCFT]2.0.CO;2

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T. Koyama,¹ Y. Kobayashi,² Y. Omata,³ M. Yamada,² H. Furuoka,² R. Maeda,¹ T. Matsui,² A. Saito,¹ T. Mikami⁴

¹Department of Veterinary Physiology, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555.
²Department of Veterinary Pathology, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555.
³To whom correspondence should be addressed.
⁴National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, Japan

Abstract

Neospora caninum was isolated from the brain of a naturally infected pregnant sheep by inoculation of immunodeficient mice with a homogenate of the brain tissue. The ewe showed no clinical signs. Tachyzoites were observed in the tissues of the nu/nu mice injected with the brain tissue homogenate and the diagnosis was confirmed by immunohistochemical staining with anti-N. caninum antibodies and by detecting N. caninum-specific DNA by polymerase chain reaction.

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T. Koyama, Y. Kobayashi, Y. Omata, M. Yamada, H. Furuoka, R. Maeda, T. Matsui, A. Saito, and T. Mikami "Isolation of Neospora caninum From the Brain of a Pregnant Sheep," Journal of Parasitology 87(6), 1486-1488, (1 December 2001). https://doi.org/10.1645/0022-3395(2001)087[1486:IONCFT]2.0.CO;2

Published: 1 December 2001

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