The helminth burdens and biodiversity of 9 randomly selected donkeys (Equus asinus) were examined after necropsy. Prior to necropsy, 8 of the animals were subjected to 1 of 3 management interventions (monthly fecal removal, prewinter moxidectin treatment, and a combination of both treatments) or a control for a 16-mo period. The remaining animal was killed earlier in the study. Quantitative samples were collected from the gastrointestinal tract for helminth recovery. The intestinal walls were examined with transmural illumination and thereafter digested with a HCl-peptic method for identification of the mucosal larval stages of cyathostomes. In this study, 37 helminth species belonging to the Ascarididae, Atractidae, Dictyocaulidae, Habronematidae, Onchocercidae, Oxyuridae, Strongylidae, and Trichostrongylidae, 1 cestode species of the Anoplocephalidae, and 1 trematode species in the Paramphistomatidae were recovered. In addition, 1 species of oestrid fly of the Gasterophilidae was identified. The results obtained in the present study support the application of several intervention methods to reduce the helminth burdens in donkeys.