The activity of ivermectin and albendazole against larval Anisakis simplex was tested in vitro and in experimentally infected guinea pigs. Before drug exposure the medium for half of the larvae was adjusted to pH 2.0 with 1 N HCl, whereas the other half was held at pH 7.0. To these solutions, ivermectin was added to full concentrations of 1, 2, 5, 10, 50, 100, or 200 μg/ml, and for albendazole, 300, 400, and 500 μg/ml. Animals from group I were given 0.1 ml of 1% (3.3 mg/kg) ivermectin, whereas guinea pigs from group II were each given 5–7 mg (16.6–23.3 mg/kg) of albendazole orally. The efficacy of both drugs against L₃ A. simplex was high in vitro and in vivo against the larvae in different organs of guinea pigs.