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1 February 2004 USE OF MONOCLONAL ANTIBODIES FOR FLOW CYTOMETRIC DETECTION OF INTRACELLULAR TOXOPLASMA GONDII IN MURINE SPLENIC LYMPHOCYTES
Eun-Hee Shin, Sung-Bum Kim, Ho-Woo Nam, Eun-Taek Han, Jae-Hwan Park, Hye-Jin Ahn, Jong-Yil Chai
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Abstract

Various monoclonal antibodies (mAbs) against Toxoplasma gondii RH tachyzoites were used for flow cytometric detection of intracellular parasites in murine splenic lymphocytes. Tg110 and Tg563 (reacting with the major surface protein SAG1), Tg505 (with another surface protein SAG2), Tg695 and Tg786 (with rhoptry proteins), Tg507, Tg621, and Tg317 (with dense granule proteins), Tg536 (with a microneme protein), and Tg685 (with a cytosol antigen) were the mAbs used. After an in vitro infection of lymphocytes with tachyzoites and reactions with the different mAbs, flow cytometry was performed using an indirect immunofluorescent technique. The proportions of whole infected lymphocytes and of each infected lymphocyte phenotype, CD4 T cells, CD8 T cells, and B cells, were determined, and their fluorescent intensities were quantified. The best reaction was seen when Tg110 or Tg695 was used as the mAbs. The results suggest that mAbs against surface or rhoptry proteins are highly useful for the flow cytometric detection of intracellular T. gondii in host cells.

Eun-Hee Shin, Sung-Bum Kim, Ho-Woo Nam, Eun-Taek Han, Jae-Hwan Park, Hye-Jin Ahn, and Jong-Yil Chai "USE OF MONOCLONAL ANTIBODIES FOR FLOW CYTOMETRIC DETECTION OF INTRACELLULAR TOXOPLASMA GONDII IN MURINE SPLENIC LYMPHOCYTES," Journal of Parasitology 90(1), 161-166, (1 February 2004). https://doi.org/10.1645/GE-3183
Received: 22 January 2003; Accepted: 1 May 2003; Published: 1 February 2004
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