Translator Disclaimer
1 August 2004 DIFFERENTIAL EFFECTS OF INTERFERON-γ ON PRODUCTION OF TRYPANOSOME-DERIVED LYMPHOCYTE-TRIGGERING FACTOR BY TRYPANOSOMA BRUCEI GAMBIENSE AND TRYPANOSOMA BRUCEI BRUCEI
Author Affiliations +
Abstract

Trypanosome-derived lymphocyte-triggering factor (TLTF) produced by Trypanosoma brucei brucei stimulates production of interferon-γ (IFN-γ) by CD8 T cells, and it is reported that, in turn, IFN-γ stimulates proliferation of T. b. brucei. We studied the role of TLTF in trypanosome proliferation using the Wellcome strain (WS) of Trypanosoma brucei gambiense and the ILtat 1.4 strain (IL) of T. b. brucei. Increase in the number of WS in infected rats is more rapid than IL and corresponds with comparatively higher levels of IFN-γ. Production of IFN-γ, as measured by protein and messenger RNA (mRNA) levels, was maintained by splenocytes from WS-infected rats, whereas levels decreased in IL-infected rats, accompanied by prolongation of infection. Expression of TLTF mRNA by in vitro–cultured WS was promoted in a dose-dependent fashion by addition of recombinant rat IFN-γ at all concentrations tested. The addition of lower concentrations of IFN-γ to cultured IL increased expression of TLTF mRNA, whereas, in contrast to WS, addition of 100 and 1,000 U/ml IFN-γ decreased expression of TLTF by IL. These results show that unlike WS, elevated IFN-γ concentrations lead to decreased TLTF production by IL. It is believed that decreased TLTF production in IL-infected rats leads to lowered IFN-γ production, thereby slowing IL proliferation.

Kazuhiko Nishimura, Kanako Hamashita, Yasuyuki Okamoto, Fumiya Kawahara, Hideshi Ihara, Shunji Kozaki, Yoshihiro Ohnishi, and Shinji Yamasaki "DIFFERENTIAL EFFECTS OF INTERFERON-γ ON PRODUCTION OF TRYPANOSOME-DERIVED LYMPHOCYTE-TRIGGERING FACTOR BY TRYPANOSOMA BRUCEI GAMBIENSE AND TRYPANOSOMA BRUCEI BRUCEI," Journal of Parasitology 90(4), 740-745, (1 August 2004). https://doi.org/10.1645/GE-211R1
Received: 7 August 2003; Accepted: 1 November 2003; Published: 1 August 2004
JOURNAL ARTICLE
6 PAGES


SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top