Hepatozoon polytopis, described from Coluber constrictor priapus from Palm Beach County, Florida, has short, usually broad gamonts 12.8 × 4.6 μm (10.0–15.0 × 3.5–6.0), with LW 58.5 μm² (42–84) and L/W 2.84 (1.8–3.7). Nuclei commonly extend into first quarter of gamont (45%), are always present in second quarter, and seldom in third quarter (11%), with dimensions 4.5 × 3.4 (3.0–6.0 × 2.5–4.5) and LW 15.1 (10.0–24.0). Spherical to ovoid oocysts, 122.1 × 104.9 (62–240 × 57–190), with L/W 1.17 (1.0–1.9), contain 31.3 (3–103) sporocysts. Spherical to ovoid sporocysts, 38.0 × 33.9 (28–73 × 25– 58), with LW 1,325.1 (756–4,168) and L/W 1.12 (1.0–1.4), contain 42.9 (22–64) sporozoites. Thamnophis sauritus sackenii from Palm Beach County is infected also by H. polytopis, as indicated from similar gamont dimensions and verified by isolation of an identical haplotype of the 18S ribosomal RNA gene from both host species.