SEQUENCE VARIATION OF THE RIBOSOMAL DNA SECOND INTERNAL TRANSCRIBED SPACER REGION IN TWO SPATIALLY DISTINCT POPULATIONS OF AMBLYOMMA AMERICANUM (L.) (ACARI: IXODIDAE)

M. V. Reichard; A. A. Kocan; R. A. Van Den Bussche; R. W. Barker; J. H. Wyckoff; S. A. Ewing

doi:10.1645/GE-292R1

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1 April 2005 SEQUENCE VARIATION OF THE RIBOSOMAL DNA SECOND INTERNAL TRANSCRIBED SPACER REGION IN TWO SPATIALLY DISTINCT POPULATIONS OF AMBLYOMMA AMERICANUM (L.) (ACARI: IXODIDAE)

M. V. Reichard, A. A. Kocan, R. A. Van Den Bussche, R. W. Barker, J. H. Wyckoff, S. A. Ewing

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J. of Parasitology, 91(2):260-263 (2005). https://doi.org/10.1645/GE-292R1

Abstract

Sequence analysis of the ribosomal DNA second internal transcribed spacer (ITS 2) region in 2 spatially distinct populations of Amblyomma americanum (L.) revealed intraspecific variation. Nucleotide sequences from multiple DNA extractions and several polymerase chain reaction amplifications of eggs from mixed-parentage samples from both populations of ticks revealed that 12 of 1,145 (1.0%) sites varied. Three of the 12 sites of variation were distinct between the 2 A. americanum populations, which corresponded to a rate of 0.26%. Phylogenetic analysis based on ITS 2 sequences provided strong support (i.e., bootstrap value of 80%) that wild A. americanum clustered into a distinguishable group separate from those derived from colony ticks.

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M. V. Reichard, A. A. Kocan, R. A. Van Den Bussche, R. W. Barker, J. H. Wyckoff, and S. A. Ewing "SEQUENCE VARIATION OF THE RIBOSOMAL DNA SECOND INTERNAL TRANSCRIBED SPACER REGION IN TWO SPATIALLY DISTINCT POPULATIONS OF AMBLYOMMA AMERICANUM (L.) (ACARI: IXODIDAE)," Journal of Parasitology 91(2), 260-263, (1 April 2005). https://doi.org/10.1645/GE-292R1

Received: 1 December 2003; Accepted: 1 July 2004; Published: 1 April 2005

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