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1 June 2005 Human Malaria Parasites in Continuous Culture
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Abstract
Plasmodium falciparum can now be maintained in continuous culture in human erythrocytes incubated at 38°C in RPMI 1640 medium with human serum under an atmosphere with 7 percent carbon dioxide and low oxygen (1 or 5 percent). The original parasite material, derived from an infected Aotus trivirgatus monkey, was diluted more than 100 million times by the addition of human erythrocytes at 3- or 4-day intervals. The parasites continued to reproduce in their normal asexual cycle of approximately 48 hours but were no longer highly synchronous. They have remained infective to Aotus.
William Trager and James B. Jensen "Human Malaria Parasites in Continuous Culture," Journal of Parasitology 91(3), (1 June 2005). https://doi.org/10.1645/0022-3395(2005)091[0484:HMPICC]2.0.CO;2
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