A Restriction Enzyme–Based Assay to Distinguish Between Avian Hemosporidians

Jon S. Beadell; Robert C. Fleischer

doi:10.1645/GE-3412RN

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Jon S. Beadell,^1,2 Robert C. Fleischer¹

¹Genetics Program, National Museum of Natural History, Smithsonian Institution, 3001 Connecticut Avenue NW, Washington, DC 20008
²Department of Biology, University of Maryland, College Park, Maryland 20742. beadellj@si.edu

Abstract

We describe a reliable and relatively inexpensive method for detecting and differentiating between the commonly studied avian blood parasite genera Haemoproteus, Plasmodium, and Leucocytozoon. The assay takes advantage of a Haemoproteus-specific restriction site identified by sequencing full mitochondrial genomes from two Haemoproteus and three Plasmodium lineages and an adjacent, genus-specific restriction site identified in Leucocytozoon spp. The assay was sensitive to simulated parasitemias of approximately 8 × 10⁻⁶ per erythrocyte and was 100% accurate in differentiating between parasite genera isolated from a broad geographical and taxonomic sampling of infected hosts.

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Jon S. Beadell and Robert C. Fleischer "A Restriction Enzyme–Based Assay to Distinguish Between Avian Hemosporidians," Journal of Parasitology 91(3), 683-685, (1 June 2005). https://doi.org/10.1645/GE-3412RN

Published: 1 June 2005

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