Genetic Characterization of Cryptosporidium Isolates From Ringed Seals (Phoca hispida) in Northern Quebec, Canada

Mónica Santín; Brent R. Dixon; Ronald Fayer

doi:10.1645/GE-3438RN

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Mónica Santín,¹ Brent R. Dixon,^1,2 Ronald Fayer^1,3

¹Environmental Microbial Safety Laboratory, Animal and Natural Resources Institute Agricultural Research Service, United States Department of Agriculture, Building 173, BARC-East, 10300 Baltimore Avenue, Beltsville, Maryland 20705
²Bureau of Microbial Hazards, Food Directorate, Health Canada, 4th floor West, Banting Research Centre, Tunney's Pasture, Ottawa, Ontario, Canada K1A 0L2
³To whom correspondence should be addressed. rfayer@anri.barc.usda.gov

Abstract

This study reports the molecular characterization of Cryptosporidium spp. isolates identified from intestinal contents of ringed seals (Phoca hispida) from Nunavik (Quebec, Canada). Cryptosporidium spp. fragments of 18S rRNA, HSP-70, and actin loci were amplified by PCR from seal intestinal contents. PCR-positive specimens were sequenced and compared with other Cryptosporidium species and genotypes reported previously. Sequence analysis showed the presence of C. muris and 2 novel genotypes in ringed seals.

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Mónica Santín, Brent R. Dixon, and Ronald Fayer "Genetic Characterization of Cryptosporidium Isolates From Ringed Seals (Phoca hispida) in Northern Quebec, Canada," Journal of Parasitology 91(3), 712-716, (1 June 2005). https://doi.org/10.1645/GE-3438RN

Published: 1 June 2005

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