Nourseothricin Acetyltransferease: A Positive Selectable Marker for Toxoplasma gondii

Tam T. Van; Peggy J. Rooney; Laura J. Knoll

doi:10.1645/GE-706R.1

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Tam T. Van,^1,2 Peggy J. Rooney,^1,2 Laura J. Knoll^1,3

¹Department of Medical Microbiology and Immunology, University of Wisconsin-Madison, 1300 University Avenue, Madison, Wisconsin 53706
²These authors contributed equally to this research note
³To whom correspondence should be addressed. ljknoll@wisc.edu

Abstract

Molecular analysis of parasite genomes will require new molecular genetic tools. The nat1 gene of Streptomyces noursei encodes nourseothricin acetyltransferase, conferring resistance to the aminoglycoside antibiotic nourseothricin. Electroporation of nat1 cassettes into RH or Prugniaud strains of Toxoplasma gondii allows for selection of stable nourseothricin-resistant clones.

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Tam T. Van, Peggy J. Rooney, and Laura J. Knoll "Nourseothricin Acetyltransferease: A Positive Selectable Marker for Toxoplasma gondii," Journal of Parasitology 92(3), 668-670, (1 June 2006). https://doi.org/10.1645/GE-706R.1

Published: 1 June 2006

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