Austrobdella coliumicus n. sp. is described from Coliumo Bay, Chile. It is characterized by a continuous, external coelomic canal (=marginal lacuna), 5 pairs of testisacs, accessory gland cells, a body not distinctly divided into trachelosome and urosome, 2 pairs of dorsal ocelli on the trachelosome, dorsal and ventral segmental ocelli present on the urosome, green overall pigmentation with transverse brown bands, and the absence of conducting tissue. Austrobdella coliumicus is distinguished from other species of Austrobdella by the presence of 2 pairs of ocelli on the trachelosome and a more cylindrical body. It is unusual that this leech was collected from inside the mantle cavity of the razor clam, Ensis macha. Austrobdella losmoliniensis n. sp. is described from the electric ray, Discopyge tschudii, collected at Los Molinos, Chile. It is characterized by a continuous, external coelomic canal; 5 pairs of testisacs; accessory gland cells; a body distinctly divided into trachelosome and urosome; 1 pair of eyes on the oral sucker; overall black pigmentation with unpigmented areas; and the absence of conducting tissue. Austrobdella losmoliniensis can be distinguished from other species of Austrobdella by the combination of 1 pair of eyes on the oral sucker and black pigmentation.