Microsatellite Markers for the Human Nematode Parasite Ascaris lumbricoides: Development and Assessment of Utility

Charles D. Criscione; Joel D. Anderson; Kyle Raby; Dan Sudimack; Janardan Subedi; Dev R. Rai; Ram P. Upadhayay; Bharat Jha§; Sarah Williams-Blangero; Timothy J. C. Anderson

doi:10.1645/GE-1058R.1

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Charles D. Criscione,¹ Joel D. Anderson,² Kyle Raby,³ Dan Sudimack,³ Janardan Subedi,⁴ Dev R. Rai,³ Ram P. Upadhayay,³ Bharat Jha§,⁵ Sarah Williams-Blangero,³ Timothy J. C. Anderson³

¹To whom correspondence should be addressed
²Perry R. Bass Marine Fisheries Research Station, Coastal Fisheries Division, Texas Parks and Wildlife Department, Palacios, Texas 77465
³Department of Genetics, Southwest Foundation for Biomedical Research, P.O. Box 760549, San Antonio, Texas 78245
⁴Department of Sociology and Gerontology, Miami University, Oxford, Ohio 45056
⁵Tribhuvan University Institute of Medicine, P.O. Box 1524, Majarajgung, Kathmandu, Nepal. ccriscio@darwin.sfbr.org

Abstract

We describe 35 microsatellite markers from the human parasitic nematode Ascaris lumbricoides. We found 7 sex-linked markers and demonstrate that 26 autosomal loci can be scored reliably. These markers have high genetic variability and provide the tools to address multiple questions concerning the epidemiology, fine-scale genetic structure, host specificity, and mating systems of this parasite.

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Charles D. Criscione, Joel D. Anderson, Kyle Raby, Dan Sudimack, Janardan Subedi, Dev R. Rai, Ram P. Upadhayay, Bharat Jha§, Sarah Williams-Blangero, and Timothy J. C. Anderson "Microsatellite Markers for the Human Nematode Parasite Ascaris lumbricoides: Development and Assessment of Utility," Journal of Parasitology 93(3), 704-708, (1 June 2007). https://doi.org/10.1645/GE-1058R.1

Published: 1 June 2007

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