Detection and Species Identification of Cryptosporidium from Taiwan Feeding Animals

Bing-Mu Hsu; Hau-Yang Wun; Chih-Li Lilian Hsu

doi:10.1645/GE-1135.1

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1 February 2008 Detection and Species Identification of Cryptosporidium from Taiwan Feeding Animals

Bing-Mu Hsu, Hau-Yang Wun, Chih-Li Lilian Hsu

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J. of Parasitology, 94(1):252-256 (2008). https://doi.org/10.1645/GE-1135.1

Abstract

In this study, 107 fecal specimens were collected from 40 sampling sites in Taiwan livestock and avian farms to test for Cryptosporidium spp. oocysts. Ten of 107 samples analyzed by enzyme-linked immunosorbent assay showed the presence of Cryptosporidium spp., among which 6 samples were simultaneously confirmed by immunofluorescence assay and polymerase chain reaction. Nucleic acid sequencing of the 18S rRNA gene identified 3 clusters of Cryptosporidium spp. Three Cryptosporidium parvum isolates were from cattle and sheep feces. One Cryptosporidium andersoni isolate was detected from pig feces. The other 2 novel Cryptosporidium genotypes were not similar to any known Cryptosporidium spp. according to the DNA sequences of the 18S rRNA gene.

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Bing-Mu Hsu, Hau-Yang Wun, and Chih-Li Lilian Hsu "Detection and Species Identification of Cryptosporidium from Taiwan Feeding Animals," Journal of Parasitology 94(1), 252-256, (1 February 2008). https://doi.org/10.1645/GE-1135.1

Received: 27 November 2006; Accepted: 1 July 2007; Published: 1 February 2008

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