Detection of Cryptosporidium parvum Oocysts by Dot-Blotting Using Monoclonal Antibodies to Cryptosporidium parvum Virus 40-kDa Capsid Protein

Mark C. Jenkins; Celia N. O'Brien; James M. Trout

doi:10.1645/GE-1313.1

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1 February 2008 Detection of Cryptosporidium parvum Oocysts by Dot-Blotting Using Monoclonal Antibodies to Cryptosporidium parvum Virus 40-kDa Capsid Protein

Mark C. Jenkins, Celia N. O'Brien, James M. Trout

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J. of Parasitology, 94(1):94-98 (2008). https://doi.org/10.1645/GE-1313.1

Abstract

Monoclonal antibodies (MAb) were prepared against the 40-kDa capsid protein of Cryptosporidium parvum virus (CPV) by immunizing mice with purified recombinant CPV40 protein. In immunoblotting analysis, MAbCPV40-1 bound to a 40-kDa protein in extracts of C. parvum oocysts. This 40-kDa protein was localized in the sporozoite cytoplasm by immunofluorescence (IFA) staining with MAbCPV40-1. In a dot-blot assay, MAbCPV40-1 was capable of detecting 10² non–bleach-treated and 10²–10³ bleach-treated C. parvum oocysts. MAbCPV40-1 was capable of detecting CPV40 antigen in both soluble and total C. parvum oocyst protein extracts, indicating a potential use for detecting this parasite in environmental samples.

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Mark C. Jenkins, Celia N. O'Brien, and James M. Trout "Detection of Cryptosporidium parvum Oocysts by Dot-Blotting Using Monoclonal Antibodies to Cryptosporidium parvum Virus 40-kDa Capsid Protein," Journal of Parasitology 94(1), 94-98, (1 February 2008). https://doi.org/10.1645/GE-1313.1

Received: 3 May 2007; Accepted: 1 July 2007; Published: 1 February 2008

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