Thirty-four amphibians (6 families, 12 species) and 28 reptiles (5 families, 16 species) from Paraguay were examined for helminths. Fifteen (44%) amphibians and 6 (21%) reptiles were found to harbor at least 1 species of helminth; 4 (12%) amphibians and 2 (7%) reptiles harbored multiple infections. Three species of Cestoidea and 17 species of Nematoda were found in the herptiles surveyed. Fourteen new host and 12 new locality records are documented, including the first report of the filaroid nematode, Macdonaldius grassi (Caballero, 1954) Chabaud and Frank, 1961, from South America.